Isolasi dan Identifikasi Bakteri Termofilik Penghasil Enzim Selulase dari Sumber Air Panas Sila

Laury M. Ch. Huwae, Pingkan Aditiawati

Abstract


Cellulose is included in the main polysaccharide group that is widely available in nature. Cellulose is a straight-chain glucose polymer that is bound by β-1,4 glucoside bonds. Cellulase is an enzyme complex consisting of several enzymes that work in stages or together in breaking down cellulose into glucose, by hydrolyzing the β-1,4 glucoside bond in cellulose. Cellulase enzymes have been used in various industries. Industrial applications require enzymes that are stable at high temperatures. Various types of thermostable enzymes can be isolated from thermophilic bacteria, cellulase enzymes are no exception. This study aims to isolate and identify thermophilic bacteria that produce cellulase enzymes from Sila hot springs. The results of isolation and screening of cellulase-producing bacteria from hot water samples obtained by 7 isolates that have the ability to degrade cellulose can produce clear zones on LB-CMC agar selective medium, but CR2 isolates showed the largest zone of 1.5 cm. CR2 isolates were Gram-positive bacteria and were identified as having close relations with Bacillus aryabhattai strain B8W22 using the 16S rRNA method.

Keywords: Cellulose; Cellulose enzyme; Thermostable enzyme; Thermophilic bacteria


Full Text:

PDF

References


Alnahdi, H.S. 2012. Isolation and screening of extracellular proteases produced by new isolated Bacillus sp. Journal of Applied Pharmaceutical Science, 2(9), 071–074.

Hartanti. 2010. Termofilik dari Kawah Air Panas. Skripsi. Institut Pertanian Bogor, Bogor.

Mussatto, S.I., and Teixeira, J.A. 2010. Lignocellulose as Raw Material in Fermentation Processes. In: Villas, A.M. (ed.), Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Formatex Research Center, pp. 897–907.

Nanda, P.T., Siregar, S.A., Kurniawan, R., Hairuidin, Meriyanti, dan Yatno. 2017. Isolasi, Karakterisasi dan Uji Potensi Bakteri Penghasil Enzim Termostabil Air Panas Kerinci. Chempublish Journal, 2(1), 26–31.

Nenci. 2012. Isolasi dan Karakterisasi Selulase dari Trichoderma viride Strain T051 dengan Substrat Jerami. Skripsi. Universitas Indonesia, Depok

Shuangqi, T., Zhenyu W., Ziluan F., Lili Z., and Jichang, W. 2011. Determination methods of cellulase activity. African Journal of Biotechnology, 10(37), 7122–7125.

Standards Unit. 2019. UK Standards for Microbiology Investigations. Bacteriology-Test Procedures, TP 30, Issue no. 4, 1–13.




DOI: https://doi.org/10.31957/acr.v3i1.1214

Refbacks

  • There are currently no refbacks.