Uji Efektivitas Metode Isolasi DNA Genom Kopi Arabika (Coffea arabica L.) Asal Kabupaten Jayawijaya

https://doi.org/10.31957/jbp.10

Authors

  • Arsyam Mawardi PS. Biologi, Jurusan Biologi, FMIPA Uncen
  • Maria L. Simonapendi Jurusan Biologi FMIPA Uncen

Abstract

Genetic substance of DNA has many functions as a basic component of the organism. DNA can be obtained directly through the isolation of DNA. Isolation of genomic DNA Wamena arabica coffee is done by treating the young leaves to get DNA extract. This reasearch is intended to provide scientific contributions in an effort to screen the best methods of DNA isolation, including a modified extraction of Zheng et al method (2005), a modified method of Doyle and Doyle (1990), and method of George and Khan modificaation (2008) or CTAB method. All of methods were tested on Arabica coffee Jayawijaya Wamena. Testing was done by looking at the difference in quality and quantity of products in the form of genomic DNA concentration and DNA thickness, comparing with the marker DNA then electrophoresis on agarose gel. From the results of testing the effectiveness of three types of isolation methods, it was found that the method of George and Khan (2008) or CTAB genomic DNA  produce the best quality than other methods. In terms of quantity, the criteria in the form of DNA concentrations ranging from 100 ng λ-DNA/ml, λ-DNA 50 ng/ml, λ-DNA 10 ng/ml. Concentration of genomic DNA bands visible when the profile is visualized in gel electrophoresis with UV luminescence. This study will be a step and initial information about the genetic composition of a population of arabica coffee which still exists, and will be developed through DNA amplification technique. 

Key words: coffee, isolation of genomic DNA, CTAB method, profile band pattern

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Author Biography

Arsyam Mawardi, PS. Biologi, Jurusan Biologi, FMIPA Uncen

Jurusan Biologi FMIPA Uncen

References

Aga, E. 2005. Molecular genetic diversity study of forest coffee tree (Coffea arabica L.) populations in Ethiopia: implications for conservation and breeding. Swedish University of Agricultural Sciences. Alnarp. Sweden.

Alatar, A.A., M.A. Mahmoud, S.A. Al-Sohaibani and K.A. Abd-Elsalam. 2012. Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants. Genetics and Molecular Research. 11(1): 348–354.

Anonimous. 2010. Perjalanan panjang Wamena Coffee. P.T. Toarco Jaya. Makassar.

Diniz, L.E.C., C.F. Ruas, V.P. Carvalho, F.M. Torres, E.A. Ruas, M.O. Santos, T. Sera and P.M. Ruas. 2009. Genetic diversity among 40 coffee varieties accessed by RAPD markers associated with restriction digestion. Arq Biol tecnol. 4: 511–521.

Faatih, M. 2009. Isolasi dan digesti DNA kromosom. Jurnal Penelitian Sains dan Teknologi. 10(1): 61–67.

George, I.A. Khan, F.S. Awan, A. Ahmad and A.A. Khan. 2008. A modified mini-prep method for economical and rapid extraction of genomic DNA in plants. Plant Molecular Biology Reporter. 22: 89a-89e.

Lashermes, P., M.C. Combes, J. Cros, P. Trouslot, F. Anthony, and A. Charrier. 2011. Origin and genetik diversity of Coffea arabica L. based on DNA molecular markers. Asic Colleque Kyoto. 6: 528–537.

Hue, T.T.M., 2005. Genetic variation in cultivated coffee (Coffea arabica L.) accessions New South Wales. Australia.

Silvestrini, M., M.G. Junqueira, A.C. Favarin, O.G. Filho, M.P. Maluf, M.B. Silvarolla and C.A. Colombo. 2007. Genetic diversity and structure of Ethiopian, Yemen and Brazilian Coffea arabica L. accession using microstellit markers. Genet Resour Crop Evol. 54: 367–379.

Specialty Coffe Association of Indonesia (SCAI). 2010. Produksi dan pemasaran. (http://www.sca-indo.or/id).

Steiger, D.L., M. Noirot and R. Ming. 2007. AFLP Analysis of genetic diversity within and among coffea arabica varities. Theor Appl Genet. 105: 209–215.

Thieman, W.J., and A.P. Michael. 2013. Introduction to Biotechnology. Pearson: New York, USA.

Utami A, R. Meryalita, N.A. Prihatin, L. Ambarsari, P.A. Kurniatin, and W. Nurcholis. 2012. Variasi metode isolasi DNA daun temulawak (Curcuma xanthorrhiza Roxb). Prosiding Seminar Nasional Unesa. Surabaya.

Vieira, S.N., V. Pinho, M.G.G. Carvalho, D.G. Esselink and B. Vosman. 2010. Development of microsatellite marker for identifying Brazilian coffea arabica varietas. Genetic and Molecular Biology online Ahead of print. 20: 12–14.

Yuwono, T. 2006. Bioteknologi Pertanian. Gadjah Mada University Press. Yogyakarta.

Zheng, H., J. Zhang, J. Li, and S. Cai. 2005. Effects of mg2+ on supported bilayer membrane on a glassy carbon electrode during membrane formation. International Journal of Electrochemical Science. 2: 788–796.

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Published

2018-05-13

Issue

Vol. 8 No. 1 (2016)

Section

Research Articles

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