Aktivitas Antibakteri Ekstrak Etanol Daun Gatal (Laportea aestuans (L.) Chew)

https://doi.org/10.31957/jbp.101

Authors

  • Eva S. Simaremare Jurusan Farmasi, FMIPA Universitas Cenderawasih
  • Agustina Ruban Jurusan Farmasi, FMIPA Universitas Cenderawasih
  • Dirk Y.P. Runtuboi Biology, Cenderawasih University

Abstract

Daun gatal (Laportea aestuans) is an indigenous plant of Papua hich has been widely used for pain relief as traditional medication in the local community. The leaves were just picked then treated to cure for painful body. This treatment are giving itchy sense as an indication that medication is working on the body as local people assumed. The aim of the study was to determine the activity of antibacteria of daun gatal against Escherichia coli, Staphylococcus aureus and Salmonella typhi. Samples of daun gatal were collected from Biak Numfor Papua. The methods are started with filtering of daun gatal using 100 mash and extracted with ethanol. Extract was made in 250, 500, 750, 1000 ppm, with chloramphenicol as a control positive, while the test of antibacterial activity was done by disc diffusion method. The results showed that daun gatal has antibacterial activity against E. coli, S. aureus and S. typhi. Daun gatal extract (L. aestuans) is effective in inhibiting the growth of bacterias with zone inhibition of 1000 ppm extracts which were 8.55 mm (E. coli), 9.02 mm (S. typhi), dan 9.37 mm (S. aureus). 

Key words: Antibacteria, E. coli, L. aestuans, S. aureus and S. typhi.

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Author Biographies

Eva S. Simaremare, Jurusan Farmasi, FMIPA Universitas Cenderawasih

Biopharmacy

Agustina Ruban, Jurusan Farmasi, FMIPA Universitas Cenderawasih

Biopharmacy,

Dirk Y.P. Runtuboi, Biology, Cenderawasih University

Biology

References

Akbar, E., and A. Malik. 2002. Antimicrobial triterpenes from Debregeasia salicifolia. Natural Product Letters. 16(5): 339-344.

Akoachere, J.F.T., Y. Suylika, A.J. Mbah, A.G. Ayimele, J.C.N. Assob, S.P.C. Fodouop, N. Kodjia, and D. Gasting. 2015. In vitro antimicrobial activity of agents from Spilanthes filicaulis and Laportea ovalifolia against some drug resistant bacteria. British Journal of Pharmaceutical Research. 6(2): 76-87.

Ariyanti, N.K., I.B.G. Darmayasa, dan S.K. Sudirga. 2012. Daya hambat ekstrak kulit daun lidah buaya (Aloe barbadensis Miller) terhadap pertumbuhan bakteri Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 25922. Universitas Udayana.

Backer, C.A., and Bakhuizen van den Brink Jr., R. C. 1965. Flora of Java. Vol. 2. Groningen: N. V . P. Noordhoff.

Davis, W.W., and T. Stout. 1971. Disc plate methods of microbiological antibiotik assay. Microbiology. 22(4): 659-665.

Dwidjoseputro. 1994. Dasar-dasar mikrobiologi. Penerbit Djambatan. Jakarta.

Hastari, R. 2012. Uji aktivitas antibakteri ekstrak pelepah dan batang tanaman pisang Ambon (Musa paradisiacal var. sapientum) terhadap Staphylococcus aureus. KTI. Universitas Diponegoro, Semarang.

Heyne, K. 1950. De nuttige planten van Indonesie. 3rd ed. S’Gravenhage & Van Hoeve. Bandung.

Heyne, K. 1987. Tumbuhan berguna Indonesia II. Badan Litbang Kehutanan. Jakarta.

Holle, E., E.S. Simaremare, Y.R. Yabansabra, E. Gunawan, dan A. Ruban. 2016. Uji mutu fisik sediaan salep daun gatal (Laportea decumana (Roxb.) Wedd.) sebagai kandidat antinyeri. Galenika. 2(3). (accepted).

Holle, E., Y.R. Yabansabra , I,M. Budi, E.S. Simaremare, E. Gunawan, A. Ruban, dan G. Wabiser. 2015. Evaluasi, uji aktivitas dan pengembangan produk salep daun gatal varietas Biak. Prosiding Seminar Biologi Nasional Papua ke-XXI. Jayapura. September, 2015. Hal: 412-419.

Jawetz, E., JM and E.A. Adelberg. 2005. Mikrobiologi untuk profesi kesehatan. Penerbit EGC Buku Kedokteran. Jakarta.

Kan, Y., I. Orhan, U. Koca, B. Özçelik, S. Aslan, M. Kartal, and S.

Küsmenoglu. 2009. Fatty acid profile and antimicrobial effect of the seed oils of Urtica dioica and U. pilulifera Turkish. Journal of Pharmaceutical Sciences; 6(1): 21-30.

Kavalali, G. 2003. The chemical and pharmacological aspects of Urtica. Taylor and Francis. Ltd. London.

Mariani, R., E.Y. Sukandar, and A.G. Suganda. 2014. Antimicrobial activity from Indonesia Urticaceae. Internasional Journal of Pharmacy and Pharmaceutical Sciences. 6(4): 191-193.

Mom, S.A, M.A. Langi, R.P. Kainde, dan W. Nurwawan. 2016. Studi etnobotani daun gatal di Kecamatan Kwamkilama Kabupaten Mimika. Ilmu Kehutanan Universitas Samratulangi. Manado. Diakses 3 Maret 2017.

Oloyede, K.G., and O.E. Ayanbadejo. 2012. Phytochemical, toxicity, antimicrobial, and antioksidan screening of extract obtain from Laportea aestuans (Gaud). J. Med Sci. 14(2): 51-59.

Oloyede, G.K. 2016. Toxicity, antimicrobial and antioxidant activities of methyl salicylate dominated essential oils of Laportea aestuans (Gaud.). Arabian Journal of Chemistry. 9(1): 840-845.

Perdana, B.Y., A.P. Putra, dan A. Primanisa. 2016. Uji toksisitas daun jelatang (Laportea sinuata Blume.) terhadap larva nyamuk Aedes aegipty. Universitas Andalas. Diakses 4 Maret 2017.

Poelangan, M., dan P. Praptiwi. 2010. Uji aktivitas antibakteri ekstrak kulit buah manggis (Garcinia mangostana Linn). Media Penelitian dan Pengembangan Kesehatan. 20(20): 65-69.

Pratiwi, S.T. 2010. Mikrobiologi farmasi. Penerbit Erlangga. Jakarta.

Rahman, M.M., A. Khan, and M.E. Haque. 2008. Antimicrobial and citotoksik activities of Laportea crenulata. Fitoterapia. 79(7-8): 584-586.

Rios, J.L. and M.C. Recio. 2005. Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology. 100 (1-2): 80-84.

Simaremare, E. 2014. Skrining fitokimia ekstrak etanol daun gatal (Laportea decumana (Roxb.) Wedd. Pharmacy. 11(1): 98-107.

Simaremare E.S., A. Ruban, M. Nainggolan, C. Yenusi, G. Wabiser, dan E. Gunawan. 2014. Pemanfaatan daun gatal (Laportea decumana (Roxb.) Wedd.) Var. Biak sebagai antinyeri. Prosiding Seminar Nasional Biologi Papua. Jayapura, Halaman: 190-195.

Simaremare, E.S., E. Holle, Y.R. Yabansabra, I.M. Budi., dan E. Gunawan. 2015. Analisis perbandingan efektivitas antinyeri salep daun gatal dari simplisia Laportea Aestuans (L) Chew dan Laportea decumana (Roxb) Wedd. Pharmacy. 12(1): 1-10.

Tchinda. C.F., I.K. Voukeng, V.P. Beng, and V. Kuete. 2016. Antibacterial activities of the methanol extracts of Albizia adianthifolia, Alchornea laxiflora, Laportea ovalifolia and three other Cameroonian plants against multi-drug resistant Gram-negative bacteria. Saudi Journal of Biological Sciences. 6(388): 1-9.

Tualeka, S. 1986. Pemeriksaan farmakognostik dan usaha skrining komponen secara kromatografi lapis tipis daun gatal (Laportea decumana (Roxb.) Wedd) asal Maluku. [Skripsi]. Universitas Hasanuddin. Makasar.

WHO (World Health Organization). 2009. Medicinal plants in Papua New Guinea. World Health Organization, regional office for the Western Pacific. Manila.

Winduo, S. E. 2003. Indigenous knowledge of medicinal plants in Papua New Guinea. University of Canterbury. Canterbury.

Yasni dan Puro. 2012. Kajian aktivitas antibakteri daun gatal (Laportea Decumana (Roxb.) Wedd.) dan daun benalu cengkeh. [Skripsi]. Institut Pertanian Bogor.

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Published

2018-05-14

Issue

Vol. 9 No. 1 (2017)

Section

Research Articles

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