Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

https://doi.org/10.31957/jbp.1096

Authors

  • Verena Agustini Universitas Cenderawasih
  • Irma Rahayu Universitas Cenderawasih
  • Leonardo A. Numberi Universitas Cenderawasih
  • Ziyadatun Ni’mah Universitas Cenderawasih

Abstract

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.

Keywords : Chitosan, orchid, Vacin and Went, plantlet.

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Author Biographies

Verena Agustini, Universitas Cenderawasih

Biology

Irma Rahayu, Universitas Cenderawasih

Laboratorium Kultur Jaringan Tumbuhan Jurusan Biologi FMIPA Universitas Cenderawasih

Leonardo A. Numberi, Universitas Cenderawasih

Universitas Cenderawasih

Ziyadatun Ni’mah, Universitas Cenderawasih

PS Biologi FMIPA Universitas Cenderawasih

References

Bangun, H., S. Tandiono and A. Arianto. 2018. Preparation and evaluation of chitosan-tripolyphosphate nanoparticles suspension as an antibacterial agent. Journal of Applied Pharmaceutical Science 8(12): 147-156.

Chandrkrachang, S. 2002. The applications of chitin and chitosan in agriculture in Thailand In: K. Suchiva, S. Chundrakrachang, P. Methacanon, M.G. Peter (Eds). Advances in Chitin Science. 5: 458–462.

Chibu, H., and H. Shibayama. 2001 Effects of chitosan applications on the growth of several crops, In: T. Uragami., K. Kurita, T. Fukamizo (Eds). Chitin and chitosan, chitin and chitosan in life science. Yamaguchi. pp. 235–239.

Charoenwattana, P., and U. Petprapai. 2013. Effect of chitosan and lotus extracts as growth promoter in Dendrobium orchid. International Journal of Environmental and Rural Development. 4: 133-137.

Devlieghere, F., A. Vermeulen, and J. Dehevere. 2004. Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 21(6): 703-714.

Dewanty, R. 2011. Aplikasi penggunaan Chitosan terhadap pembentukan protocorm like body (PLB) pada anggrek Phalaenopsis sp.L. Skripsi. Fakultas Pertanian, Universitas Jember.

Hirano, S. 1997. Application of chitin and chitosan in the ecological and environment fields. In: FMA Goosen (Ed). Application of chitin and chitosan. Technomic Publishing Company. pp: 31-54.

Kumari, S., J. Singh and F.G. Panj. 2017. Economic returns and enhanced quality in orchid (Dendrobium Sonia 17) using biosafe compound chitosan. Agricultural Research & Tecnology. 6(3): 0050-0054.

Millar, A. 1978. Orchids of Papua New Guinea. Australian National University Press. Canberra, Australia.

Noerdjito, M. dan I. Maryanto. 2001. Jenis-jenis hayati yang dilindungi Perundang-undangan Indonesia. Puslitbang Biologi LIPI. Hal.: 151–154.

Pitoyo, A., M.R. Hani, and E. Anggarwulan. 2015. Application of chitosan spraying on acclimatization success of tiger orchid (Grammatophyllum scriptum) plantlets. Nusantara Bioscience. 7(2): 185–191.

Pospieszny, H., S. Chirkov, and J. Atebekov. 1991. Induction of antiviral resistance in plants by chitosan. Plant Sci. 79: 63–68.

Pornpienpakdee, P.R. Singhasurasak, P. Chaiyasap, R. Pichyangkura, R. Bunjongrat, S. Chadchawan and P. Limpanavech. 2010. Improving the micropropagation efficiency of hybrid Dendrobium orchids with chitosan. Scientia Horticulturae. 124: 490–499.

Samarfard, S., M.A. Kadir, S.B. Kadzimin, H.M. Saud, S.A. Ravanfar, and M. Danase. 2014. In vitro propagation and detection of somaclonal variation in Phalaenopsis gigantean as affected by chitosan and thidiazuron combinations. Hort. Science. 49(1): 1-7.

Sivanesan, I. and S.W. Park. 2014. The role of silicon in plant tissue culture. Frontiers in Plant Science. 5(571): 1-4.

Sukewijaya, I.M. 2013. Efikasi chitosan untuk memperpanjang flower longevity bunga anggrek Dendrobium hibrida dalam pot (potted flower). Agrotrop.3(2): 85-91.

Sukwattanasinitt, M., A. Klaiherd, K. Skulnee, and S. Aiba. 2001. Chitosan as a releasing device for 2,4D herbicide In: T. Uragami, K. Kurita, T. Fukamizo (Eds). Chitin and chitosan, chitin and chitosan in life ccience. Yamaguchi. pp. 142-143.

Utami, E.S.W., S. Hariyanto, and Y.S.W. Manuhara. 2017. In vitro propagation of the endangered medicinal orchid Dendrobium lasianthera J.J.Sm. through mature seed culture. Asian Pasific Journal of Tropical Biomedicine. 7(5): 406-410.

Wanichpongpan, P., K. Suriyachan, and S. Chandrkrachang. 2001. Effects of chitosan on the growth of gerberra flower plant (Gerberra jamesonii), In: T. Uragami., K. Kurita, T. Fukamizo (Eds). Chitin and chitosan, chitin and chitosan in life science. Yamaguchi. pp.: 198–201.

Wiyatie, Muslimin, dan Dewi. 2018. Pertumbuhan protocorm like bodies anggrek Coelogyne celebensis J.J.Smith pada berbagai konsentrasi air kelapa secara in vitro. Jurnal Warta Rimba 6(3): 33–41.

Wulandari, T dan D. Sukma. 2014. Karakterisasi morfologi dan pertumbuhan populasi plantlet anggrek Phalaenopsis hasil persilangan selama tahap aklimatisasi. J. Hort. Indonesia. 5(3): 137-147.

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Published

2020-03-05

Issue

Vol. 12 No. 1 (2020)

Section

Research Articles

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