Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat

Authors

  • Wahyu Irawati Universitas Pelita Harapan
  • Aaron Hasthosaputro Jurusan Biologi, Fakultas Sains dan Matematika, Universitas Pelita Harapan
  • Lucia Kusumawati International University Liaison Indonesia, Department of Food Technology

DOI:

https://doi.org/10.31957/jbp.1207

Abstract

The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS).  It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore,  it is also resistant to  heavy metal mixture of 4.5 mM.  The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively.  In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics.  

Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri

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Author Biographies

Wahyu Irawati, Universitas Pelita Harapan

Program Studi Pendidikan Biologi, Fakultas Keguruan Ilmu Pendidikan, Universitas Pelita Harapan, Tangerang

Aaron Hasthosaputro, Jurusan Biologi, Fakultas Sains dan Matematika, Universitas Pelita Harapan

Jurusan Biologi, Fakultas Sains dan Matematika, Universitas Pelita Harapan

Lucia Kusumawati, International University Liaison Indonesia, Department of Food Technology

International University Liaison Indonesia, Department of Food Technology

References

Abbas, S.Z., M. Rafatullah, K. Hossain, N. Ismail, H.A. Tajarudin, and H.P.S.A. Khalil. 2018. A review on mechanism and future perspectives of cadmium-resistant bacteria. International Journal of Environmental Science and Technology. 15(1): 243-262.

Aransiola, E.F., O.A. Ige, E.O. Ehinmitola, and S.K. Layokun. 2017. Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil. Afr. J. Biotechnol. 16(19): 1098-1105.

Banvalvi, G. 2011. Cellular effects of heavy metals. Springer. London.

Baz, S.E., M. Baz, M. Barakate, L. Hassani, A.E. Gharmali, and B. Imziln. 2015. Resistance to and accumulation of heavy metals by Actinobacteria isolated from abandoned mining areas. The Scientific World Journal. 2015: 1-14.

Chan, K.G., S. Atkinson, K. Mathee, C.L. Koh, and P. William. 2011. Characterization of N-acylhomoserine lactonedegrading bacteria associated with Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia. BMC Microbiol. 11(1): 51.

Chen, Z., X. Pan, H. Chen, Z. Lin, and X. Guan. 2015. Investigation of lead (II) uptake by Bacillus thuringiensis 016. World J. Microbiol Biotechnol. 31: 1729–1736.

Cooksey, D., and H. Azad. 1992. Accumulation of copper and other metals by cooper-resistant plant-pathogenic and saprophytic pseudomonads. Applied and Environmental Microbiology. 58: 274-278.

Dash, S., H.R. Dash, and J. Chakraborty. 2016. Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants. Appl Microbiol Biotechnol. 100: 2967-2984.

Gonzales, A.G., L. S. Shirokova, O.S. Pokrovsky, E.E. Emnova, R.E. Martinez, and J.M. Santana-Casiano. 2010. Adsorption of copper on Pseudomonas aerofaciens: protective role of surface exopolysaccharides. J. Colloid Intl Sci. 350: 305-314.

Hynninen, A. 2010. Zinc, cadmium and lead resistance mechanisms in bacteria and their contribution to biosensing. [Disertasi]. University of Helsinki. Finlandia

Irawati, W., T. Yuwono, H. Hartiko, and J. Soedarsono. 2012. Molecular and physiological characterization of copper-resistant bacteria isolated from activated sludge in an industrial wastewater treatment plant in Rungkut-Surabaya, Indonesia. Microbiol J. 3: 107-116.

Irawati, W., N.P. Ompusunggu, D.N. Susilowati, and T. Yuwono. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas. 20(2): 344-349.

Kavamura, V.N., and E. Esposito. 2010. Biotechnological strategies applied to the decontamination of soils polluted with heavy metals. Biotechnol Adv. 28(1): 61-69.

Méndez, V., S. Fuentes, V. Morgante, M. Hernández, M. González, E. Moore, and M. Seeger. 2017. Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil polluted soil. J Soil Sci Plant Nutr. 17(4): 1074-1087.

Mohan, M., N. Santosh, and K. Dubey. 2013. Lead resistant bacteria: Lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicology and Environmental Safety. 98: 1-7.

Naik, M.M., A. Pandey, and S.K. Dubey. 2012. Pseudomonas aeruginosa strain WI-1 from Mandovi estuary possesses metallothionein to alleviate lead toxicity and promotes plant growth. Ecotoxicol Environ Saf. 79: 129–133.

Nies, D.H. 1999. Microbial heavy metal resistance. Appl. Microbiol Biotechnol. 51: 730-750.

Park, J.H., N. Bolan, M. Megharaj, N. Ravi, and J.W. Chung. 2011. Bacteria assisted immobilization of lead in soils: implications for remediation. Pedologist. 54: 162-174.

Pushkar, B.K., P.I. Sevak, and A. Singh. 2015. Isolation and characterization of potential microbe for bio-remediating heavy metal from Mithi river. Ann Appl Bio-sci. 2(2): 1-27

Puyen, Z.M., E. Villagrasa, J. Maldonado, E. Diestra, I. Esteve, and A. Sole. 2012. Biosorption of lead and copper by heavy-metal tolerant Micrococcus luteus DE2008. Bioresour Technol. 126: 233–237.

Saranraj, P., and D. Stella. 2012. Bioremediation of sugar mill effluent by immobilized bacterial consortium. Intl J. Res Pure Appl Microbiol. 2(4): 43-48.

Shakoori, A., and B. Muneer. 2002. Cooper-resistance bacteria from industrial effluents and their role in remediation of heavy metals in wastewater. Folia Microbiol. 47: 43-50.

Spain, A., and E. Alm. 2003. Implications of microbial heavy metal tolerance in the environment. Undergraduate Research. 2: 1-6.

Sulaimon, A.M., A.T. Odeyemi, A.A. Ogunjobi, and L.O. Ibrahim. 2014. Bioaccumulation of heavy metals using selected heavy metal tolerant organisms isolated from dumpsite leachate. Nature and Science. 12(10): 101–106.

Vicentin, R.P., J.V. Santos, C.R.G. Labory, A.M. da Costa, F.M.S. Moreira, and E. Alves. 2017. Tolerance to accumulation of cadmium, copper, and zinc by Cupriavidus necator. Rev Bras Cienc Solo. 42: e0170080.

Williams, G.P., M. Gnanadesigan, and S. Ravikumar. 2012. Biosorption and biokinetic studies of halobacterial strains against Ni2+, Al3+, and Hg2+ metal ions. Bioresour Technol. 107: 526-529.

Williams, C.L., H.M. Neu, J.J. Gilbreath, S.L.J. Michel, D.V. Zurawski, and D.S. Merrella. 2016. Copper resistance of the emerging pathogen Acinetobacter baumannii. Appl Environ Microb. 82(20): 6174-6188.

Zaki. S., and S. Farag 2010. Isolation and molecular characterization of some copper biosorped strains. Int J Environ Sci Tech. 7(3): 533-560.

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Published

2020-09-30

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Research Articles