Phytochemical Screening and Antibacterial Activity of Dendrobium from Papua Against Eschericia coli and Staphylococcus aureus

Authors

  • Supeni Sufaati Cenderawasih University
  • Verena Agustini Universitas Cenderawasih
  • Agnes E. Maryuni Cenderawasih University
  • Eva S. Simaremare Cenderawasih University

DOI:

https://doi.org/10.31957/jbp.1645

Abstract

Orchid found as one of medicinal plant in some areas, especially Dendrobium. Some species were investigated its bioactive compound, and antibacterial activity, but the information about antibacterial activity of Papuan Dendrobium species is still limited. The aims of this research were to determine the phytochemical constituents and the antibacterial activity of ethanolic extract and fraction of Dendrobium species from Papua against Escherichia coli and Staphylococcus aureus. There were three species of Dendrobium namely: D. spectabile, D. violaceoflavens, and D. antennatum used in this study. Dried simplisia was macerated using ethanol, then tested for the phytochemical content. Total ethanolic extract was fractionated with three different solvents, ethanol, ethyl acetate and hexane. Disc diffusion assay was used to examine the antibacterial activities of the total extract and the fractions. Total extract were positive for flavonoid, alkaloid and tanin, except D. spectabile stem extract only alkaloid and tannin.  No saponin was found in the plants. The ethyl acetate fraction of leaves of D. spectabile showed the highest antibacterial activity against S. aureus with diameter of inhibition zone 20.54 + 1.47 mm (strong category). Other plant extract and fraction tested had  moderate antibacterial activities against both E.coli and S aureus  with diameter zone  6-9 mm. The present work indicates that the ethyl acetate fraction of D. spectabile leaves is potential to be developed in antibacterial drug design research.  

Key words: antibacterial activity; Papua; Dendrobium; E. coli; S. aureus. 

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Author Biographies

Supeni Sufaati, Cenderawasih University

Biology

Verena Agustini, Universitas Cenderawasih

Biology

Agnes E. Maryuni, Cenderawasih University

Chemistry

Eva S. Simaremare, Cenderawasih University

Pharmacy

References

Base, B., H. Choudlhury, P. Tandon, and S. Kumaria. 2017. Studies on secondary metabolite profiling, anti-inflammatory potential, in vitro photoprotective and skin-aging related enzyme inhibitory activities of Malaxis acuminate, a threatened orchid of nitraceutical importance. Journal of Photochemistry and Photobiology. 173: 686-695.

Bi, Z.M., Z.T. Wang, L.S. Xu, and G.J. Xa. 2003. Studies on the chemical constituents of Dendrobium fimbriatum. Acta Pharm. Sinic. 38: 526-529.

Chimsook, T. 2016. Phytochemical screening, total phenolic content, antioxidant activities and cytotoxicity of Dendrobium signatum leaves. MATEC Web of Conferences. 62: 03005.

Devi, P.U., S. Selvi, D. Devipriya,S. Murugan, and S. Suja. 2009. Antitumor and antimicrobial activities and inhibition of in-vitro lipid peroxidation by Dendrobium nobile. African Journal of Biotecnology. 8: 2289-2293.

Esha, V., C. Shilpa, P. Bharat, and Neeru. 2016. Physico-chemical and phytochemical evaluation of Dendrobium macraei Lindl (whole plant). International Journal of Pharmacognosy and Phytochemical Research. 8: 1801-1811.

Ganapaty, S., M. Ramaiah, K. Yasaswini, V.K. Nuthakki, and D. Harikrishnareddy. 2013. Quantitative phytochemical estimation and evaluation of hepatoprotective activity of methanolic extract of Dendrobium ovatum (L) Kraenzl. whole plant against CCI4 induced hepatotoxicity. Journal of Pharmacognosy and Phytochemistry. 2: 113-118.

Hoque, M.M., L. Khaleda, and M. Al-Forkan. 2015. Evaluation of pharmaceutical properties on microbial activities of some important medicinal orchids of Bangladesh. Journal of Pharmacognosy and Phytochemistry. 4: 265-269.

Johnson, M., and N. Janakiraman. 2013. Phytochemical and TLC studies on stem and leaves of the orchid Dendrobium panduratum subsp. villosum Goplan & A.N.Hendry. Indian Journal of Natural Products and Resources. 4: 250-254.

Klongkumnuankarn, P., K. Basaranon, P. Chanvorachote, B. Sritularak, V. Jongbunpraset, and K. Likhitwitayawuid. 2015. Cytotoxic and antimigratory activities of phenolic compounds from Dendrobium brymerianum. Evidence-based complementary and alternatve medicine. Hindawi Publishing Co, ID 350410 : 9 pgs.

Koloidziej, V.H., and A.F. Kiderlen. 2005. Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitized RAW 264.7 cells. Phytochemistry. 66: 2016-2071.

Laurentius, N.H., R. Pratiwi,R. Susandarini, E.R.P. Wardoyo, O. Megawati, and S. Handayani. 2016. Isolation of bioactive compounds from two orchid species and preliminary test of their cytotoxicity against T47D breast cancer cells. International Journal of Pharmacognocy and Phytochemical Research. 8: 150-155.

Li, Y.M., H.Y. Wang, and G.Q. Liu. 2001. Erianin induces apoptosis in human leukemia HL-60 cells. Acta Pharmacological Sinica. 22: 1018-1022.

Lin, T.H., S,J. Chang, C.C. Chen, J.P. Wang, and L.Ti. Tsao. 2001. Two phenanthraquinones from Dendrobium monififorme. J. Nat. Prod. 64: 1084-1086.

Paudel, M.R., N. Rajbanshi, A.R. Sah, S. Acharya, and B. Pant. 2018. Antibacterial activity of selected Dendrobium species against clinically isolated multiple drug resistant bacteria. African Journal of Microbiology Research. 12: 426-432.

Paul, P., A. Chowdhury, D. Nath., and M.K. Bhattacharjee. 2013. Antimicrobial efficacy of orchid extract as potential inhibitors of antibiotic resistant strains of Escherichia coli. Asian Journal of Pharmaceutical and Clinical Research. 6: 108-111.

Phechrmeekha, T., B. Sritularak and K. Likhitwitayawuid. 2012. New Phenolic compounds from Dendrobium capillipes and Dendrobium secundum. Journal of Asian Natural Products Research. 14(8): 748-754.

Prasad, R., and B. Koch. 2014. Antitumor activity of ethanolic extract of Dendrobium formosum in T-cell lymphoma : an in vitro and in vivo study. BioMed Research International. Article ID. 753451.

Rashmi, K., S.D. Shweta, C.S. Sudeshna, P.S. Vrushala, T.R.K Prashith, and H.L. Raghavendra. 2015. Antibacterial and radical scavenging activity of selected orchids of Karnataka, India. Science,Technology and Arts Research Journal. 4: 160-164.

Rosa, M.P.G. 2010. Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. J. Med.Plants Res. 4: 492–638.

Sandrasagaran, U.M., S. Subramaniam, and V. Murugaiyah. 2014. New perspective of Dendrobium crumenatum orchid for antimicrobial activity against selected pathogenic bacteria. Pakistan Journal of Botany. 46(2): 719-724.

Singh, A., and S. Duggal. 2009. Medicinal orchids: An overview. Ethnobotanical Leaflets. 13: 351-363.

Tanogorumeatar, K., C. Chaottham, B. Sritularak, Likhitwitayawuid, and P. Chanvorachotte. 2014. Cytotoxic and antimetastatic activities of phenolic compounds from Dendrobium ellipsophyllum. Anticancer Research. 34: 6573-6580.

William, C.A. 1978. The leaf flavonoids of the leaf the Orchidaceae. Phytochemistry. 18: 803 -813.

Xue, R., Z. Fang, M. Zhang, Z. Yi, C. Wen, and T. Shi. 2013. Traditional Chinese medicine integrative database for herb molecular mechanism analysis. Nucleic Acids Res. doi: 10.1093/nar/gks1100.

Yang, C., L. Weilong, L. Zhao, and J. Cai. 2018. A new furostanol saponin from Dendrobium chrysanthum Lindl with cytotoxic activity. Natural Product Research. 17: 2461-2465.

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Published

2021-10-01

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Research Articles