Pemanfaatan Ekstrak Kasar Daun Sirih Hijau (Piper betle) dan Daun Sirih Merah (Piper crocatum) Sebagai Antimikroba Pada Daging Ayam Broiler Segar
https://doi.org/10.31957/jbp.2881
Keywords:
Green betel leaves, red betel leaves, extracts, fresh broiler chickenAbstract
Green betel plants (Piper betle) and red betel plants (Piper crocatum) are plants that found in many parts of Indonesia which can be categorized as medicinal plants. This is due to the content contained in green and red betel leaves which has phenolic compounds, such as flavonoids, tannins, and saponins. The research objective of this study was to utilize the extract of green betel leaf and red betel leaf by extracting ethanol FG 96% as an antimicrobial compound. There are 4 types of tested bacteria used in this study, they are Escherichia coli, Salmonella, S. aureus, and B. cereus. The extract concentrations used for the two types of leaf extract were 0, 5, 10, 15, and 20%. The sample used for TPC was a fresh broiler chickens, with immersion time for 0, 24, 48, and 72 hours. The total phenolic properties of the green and red betel leaf extracts were 2630.1 mg GAE/g and 6294.8 mg GAE/g, respectively. The total flavonoids possessed by the green and red betel leaf extract were 679.0 mg QE/g and 1662.9 mg QE/g, respectively. Both types of betel leaf extract can function as the best antimicrobial at a concentration of 20%. The bacteria that most resistant to green and red betel leaf extract is E. coli, and the bacteria that most sensitive to green and red betel leaf extract is S. aureus. The MIC and MBC values of Gram positive bacteria (S. aureus and B. cereus) were lower than the MIC and MBC values of Gram negative bacteria (E. coli and Salmonella).
Key words: Green betel leaves; red betel leaves; extracts; fresh broiler chicken.
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References
AOAC. 2005. Official methods of analysis of the association of official analytical chemists. AOAC Inc., Arlington.
Arifin, B. dan S. Ibrahim. 2018. Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah. 6(1): 21-29. https://doi.org/10.31629/zarah.v6i1.313
Badan Standardisasi Nasional. 2009. Standar nasional indonesia (SNI) nomor 3924:2009 tentang mutu karkas dan daging ayam. BSN. Jakarta.
Bustanussalam, D. Apriasi, E. Suhardi, dan D. Jaenudin. 2015. Efektivitas antibakteri ekstrak daun sirih (Piper betle Linn) terhadap Staphylococcus aureus ATCC 25923. Fitofarmaka. 5(2): 58-64. https://doi.org/10.33751/jf.v5i2.409
Carvalho, A.M.S., L. Heimfarth, E. W. M. Pereira, F. S. Oliveira, I. R. A. Menezes, H. D. M. Coutinho, L. Picot, A. R. Antoniolli, J. S. S. Quintans, dan L. J. Q. Junior. 2020. Phytol, a chlorophyll component, produces antihyperalgesic, anti-inflammatory, and antiarthritic effects: possible NFκB pathway involvement and reduced levels of the proinflammatory cytokines TNF-α and IL-6. Journal of Natural Products. 83(4): 1107-1117. https://doi.org/10.1021/acs.jnatprod.9b01116
Castillo, Y.P., T. C. Lima, A. R. Ferreira, C. R. Silva, R. S. Campos, J. B. A. Neto, H. I. F. Magalhaes, B. C. Cavalcanti, H. V. N. Junior, dan D. P. Sousa. 2020. Bioactivity and molecular docking studies of derivatives from cinnamic and benzoic acids. BioMed Research International. 2020: 1-13. https://doi.org/10.1155/2020/6345429
Cepeda, G.N., M. M. Lisangan, dan I. Silamba. 2019. Aktivitas antibakteri minyak atsiri kulit kayu akway (Drimys piperita Hook. f.) pada beberapa tingkat konsentrasi, keasaman (pH) dan kandungan garam. Jurnal Aplikasi Teknologi Pangan. 8(4): 149-154. https://doi.org/10.17728/jatp.4692
Dewi, S.R., B. D. Argo, dan N. Ulya. 2018. Kandungan flavonoid dan aktivitas antioksidan ekstrak Pleurotus ostreatus. Jurnal Rona Teknik Pertanian. 11(1): 1-11. https://doi.org/10.17969/rtp.v11i1.9571
Fallo, G., L. Pardosi, dan A. M. D. Cruz. 2022. Karakterisasi bakteri endofit tanaman sirih Timor (Piper betle L.) penghasil antibakteri. Jurnal Biologi Papua. 14(2): 102-108. https://doi.org/10.31957/jbp.2366
Foo, L.W., E. Salleh, dan S. N. H. Mamat. 2015. Extraction and qualitative analysis of Piper betle leaves for antimicrobial activities. International Journal of Engineering Technology Science and Research. 2: 1-8.
Hajrawati, F. M., Wahyuni, dan I. I. Arief. 2016. Kualitas fisik, mikrobiologis, dan organoleptik daging ayam broiler pada pasar tradisional di Bogor. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 4(3): 386-389.
Huongâ€, B.M. dan T. T. K. Hue. 2019. Antibacterial finishing on cotton 100 % and cvc fabrics with tannin from Piper betle extract. ‬‬Vietnam Journal of Science and Technology. â€57‬(6): 693-702.
Insanu, M., L. Marliani, dan N. P. Dinilah. 2017. Perbandingan aktivitas antioksidan dari ekstrak daun empat tanaman marga Piper. Jurnal Pharmaciana. 7(2): 305-312. https://doi.org/10.12928/pharmaciana.v7i2.6935
Kaintura, P., M. Bhandari, dan R. Kumar. 2020. Medicinal values of betel leaves and its application in food products: a review. The Pharma Innovation Journal. 9(6): 344-348.
Khadijah, A. M., Jayali, S. Umar, dan I. Samista. 2017. Penentuan total fenolik dan aktivitas antioksidan ekstrak etanolik daun samama (Anthocephalus macrophylus) asal Ternate, Maluku Utara. Jurnal Kimia Mulawarman. 15(1): 11-18. https://doi.org/10.30872/jkm.v15i1.495
Kim, H.W., Y. S. Seok, dan M. S. Rhee. 2019. Synergistic staphylocidal interaction of benzoic acid derivatives (benzoic acid, 4-hydroxybenzoic acid and β-resorcylic acid) and capric acid: mechanism and verification study using artificial skin. Journal of Antimicrobial Chemotherapy. 75(3): 1-5. https://doi.org/10.1093/jac/dkz494
Kurnia, A., F. C. Saputri, dan Hayun. 2019. Synthesis and anticancer potential of aminomethyl derivatives of methyl-substituted asymmetrical curcumin mono-carbonyl. Journal of Applied Pharmaceutical Science. 9(8): 18-24. https://doi.org/10.7324/JAPS.2019.90803
Lubis, R.R., Marlisa, dan D. D. Wahyuni. 2020. Antibacterial activity of betle leaf (Piper betle L.) extract on inhibiting Staphylococcus aureus in conjunctivitis patient. American Journal of Clinical and Experimental Immunology. 9(1): 1-5.
Moo, C.L., S. K. Yang, M. A. Osman, M. H. Yuswan, J. Y. Loh, W. M. Lim, S. H. E. Lim, dan K. S. Lai. 2020. Antibacterial activity and mode of action of β-caryophyllene on Bacillus cereus. Polish Journal of Microbiology. 69(1): 49-54. https://doi.org/10.33073/pjm-2020-007
Mufrod, Suwaldi, dan S. Wahyuno. 2016. Patch ekstrak daun sirih (Piper betle L.): pengaruh penambahan release enhancer substances terhadap sifat fisikokimia dan aktivitas antibakteri. Majalah Farmaseutik. 12(2): 431-442.
Muruganandamâ€, L., A. Krishna, J. Reddy, dan G. S. Nirmala. 2017. Optimization†‬studies†on†‬extraction†‬of†phytocomponents†‬from†betel leaves. Resource Efficient†Technologies. 3: 385-393.https://doi.org/10.1016/j.reffit.2017.02.007.
Mustamin, M. I., N. Rustam, dan K. Kasman. 2016. Analisis nilai absorbansi kadar flavonoid daun sirih merah (Piper crocatum) dan daun sirih hijau (Piper betle L). Gravitasi. 15(1): 1-8.
Nejad, S.M., H. ÖZGÜNEŞ, dan N. Basaran. 2017. Pharmacological and toxicological properties of eugenol. Turkish Journal of Pharmaceutical Sciences. 14(2): 201-206. https://doi.org/10.4274/tjps.62207.
Noriega, P., J. Ballesteros, A. D. L. Cruz, dan T. Veloz. 2020. Chemical composition and preliminary antimicrobial activity of the hydroxylated sesquiterpenes in the essential oil from Piper barbatum Kunth leaves. Journal Plants. 9(2): 1-9. https://doi.org/10.3390/plants9020211.
Nowshad, F., M. D. Islam, dan M. S. Khan. 2018. Analysis of the concentration and formation behavior of naturally occurring formaldehyde content in food. International Journal of Food Engineering. 4(1): 71-75. https://doi.org/10.18178/ijfe.4.1.71-75.
Pangaribuan, B.B.P., T. U. Soleha, dan M. R. Ramadhian. 2019. Perbandingan daya hambat konsentrasi ekstrak etanol daun sirih hijau (Piper betle L.) terhadap pertumbuhan bakteri Salmonella typhi dan Staphylococcus aureus. J Agromedicine. 6(2): 400-404.
Pangesti, R.D., E. Cahyono, dan E. Kusuma. 2017. Perbandingan daya antibakteri ekstrak dan minyak Piper betle L. terhadap bakteri Streptococcus mutans. Indonesian Journal of Chemical Science. 6(3): 271-278.
Prayitnâ€oâ€, S.A., J. Kusnadi, dan E. S. Murtini. 2018. ‬Karakteristik (total flavonoid, total fenol, aktivitas antioksidan) ekstrak serbuk daun sirih merah â€(â€Piper crocatum Ruiz & Pav.). Foodscitech. 1(2): 26-
Puspita, P.J., M. Safithri, dan N. P. Sugiharti. 2018. Antibacterial activities of sirih merah (Piper crocatum) leaf extracts. Current Biochemistry. 5(3): 1-10. https://doi.org/10.29244/cb.5.3.1-10
Putri, R.M.S., Nurjanah, dan K. Tarman. 2018. Analisis kuantitatif mikrobiologi serbuk minuman fungsional lintah laut (Discodoris sp.) pada suhu yang berbeda selama penyimpanan. A Scientific Journal. 35(3): 124-130.
Ramadhani, W.M., I. Rukmi, dan S. N. Jannah. 2020. Kualitas mikrobiologi daging ayam broiler di pasar tradisional banyumanik semarang. Jurnal Biologi Tropika. 3(1): 8-16.
Retnaningsih, A., A. M. Ulfa, dan D. M. Khomsatun. 2018. Uji daya hambat anti bakteri infusa daun sirih merah (Piper crocatum Ruiz & Pav) dan daun sirih hijau (Piper betle L.) terhadap bakteri Staphylococcus aureus dengan metode difusi. Jurnal Analis Farmasi. 3(1): 79-88.
Shinsetsu, A.S., V. M. Aditya, dan S. Fauziyah. 2015. Sintesis eugenol menjadi 2-metoksi-4-(1-propenil) fenol melalui reaksi isomerisasi dan aplikasinya sebagai bahan suplemen pada mouthwash. Jurnal Sains dan Teknologi. 13(2): 87-92.
Sitarek, P., P. Rijo, C. Garcia, E. Skala, D. Kalemba, A. J. Bialas, J. Szemra, D. Pytel, M. Toma, H. Wysokinskaska, dan T. Sliwinski. 2017. Antibacterial, anti-inflammatory, antioxidant, and antiproliferative properties of essential oils from hairy and normal roots of Leonurus sibiricus L. and their chemical composition. Oxidative Medicine and Cellular Longevity. 2017: 1-12. https://doi.org/10.1155/2017/7384061
Soelama, H.J.J., B. J. Kepel, dan K. V. Siagian. 2015. Uji minimum inhibitory concentration (MIC) ekstrak rumput laut (Eucheuma cottonii) sebagai antibakteri terhadap Streptococcus mutans. Jurnal e-GiGi (eG). 3(2): 374-379. https://doi.org/10.35790/eg.3.2.2015.9630.
Soleha, T.U., N. Carolia, dan S. W. Kurniawan. 2015. The inhibition test of red betel leaves (Piper crocatum) towards Staphylococcus aureus and Salmonella typhi. J. Majority. 4(5): 117-122.
Suryani,Y., L. W. Sophia, T. Cahyanto, dan I. Kinasih. 2015. Uji aktivitas antibakteri dan antioksidan infusum cacing tanah (Lumbricus rubellus) dengan tambahan kitosan udang pada Salmonella thypi. Jurnal Istek. 9(2): 264-281.
Utami, M.R., I. Batubara, dan L. K. Darusman. 2017. Isolasi minyak atsiri daun sirih merah (Piper cf. fragile. Benth). Jurnal Agrotek Indonesia. 2(1): 39–43. https://doi.org/10.33661/jai.v2i1.719.
Wahjuni, S., I. W. Wita, dan I. N. M. Astawa. 2016. Anti-inflammatory effect of red Piper Crocatum leaves extract decrease TNF-α and IL-6 levels in wistar rat with atherosclerosis. Bali Medical Journal. 5(2): 240-244. https://doi.org/10.15562/bmj.v5i2.210.
Yunita, M., Y. Hendrawan, dan R. Yulianingsih. 2015. Analisis kuantitatif mikrobiologi pada makanan penerbangan (Aerofood ACS) Garuda Indonesia berdasarkan TPC (total plate count) dengan metode pour plate. Jurnal Keteknikan Pertanian Tropis dan Biosistem. 3(3): 237-248.
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