Skrining Fitokimia dan Uji Aktivitas Sitotoksik dari Tumbuhan Sarang Semut (Myrmecodia beccarii Hook.f.) Asal Kabupaten Merauke

https://doi.org/10.31957/jbp.41

Authors

  • Modustriati P. Mardany Pharmacy, Cenderawasih University
  • Linus Y. Chrystomo Biology, Cenderawasih University
  • Aditya K. Karim Biology, Cenderawasih University

Abstract

Sarang semut (Myrmecodia beccarii Hook.f.) is a famous medicinal plant of Papua, which has traditionally potential in treating a wide variety of mild and severe illnesses, such as cancers and tumors, gout, coronary heart disease, hemorrhoids, tuberculosis, migraines, rheumatism, and leukemia. The purposes of this study were to determine the content of the active compound group and the cytotoxic activity of sarang semut plants from Merauke Region. The extraction was done by maceration using 96 % ethanol. The concentration of ethanolic extract were 0, 25, 50, 75, 100 ppm. The results of the study showed that the cytotoxic activity determine using BSLT (Brine Shrimp Lethality Test) method have a high cytotoxic activity with LC50 values of 22.86 ppm and containing the active compound namely flavonoids, tannins and saponins separately. The ethanolic extract of tuber of sarang semut (M. beccarii) has a high cytotoxic activity against larvae of Artemia salina Leach according BSLT method. 

Key words: Active compound, M. beccarii, cytotoxic activity, Merauke.

Downloads

Download data is not yet available.

Author Biographies

Modustriati P. Mardany, Pharmacy, Cenderawasih University

Pharmacy, Biopharmacy

Linus Y. Chrystomo, Biology, Cenderawasih University

Pharmacy

Aditya K. Karim, Biology, Cenderawasih University

Biology

References

Bustanussalam. 2010. Penentuan struktur molekul dari fraksi air tumbuhan “sarang semut†Myrmecodia pendens Merr & Perry yang mempunyai aktivitas sitotoksik dan antioksidan. [Tesis]. Institut Pertanian Bogor, Bogor.

Cahyadi, R. 2009. Uji toksisitas akut ekstrak etanol buah pare (Momordica charantia L.) terhadap larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BST). [Skripsi]. Universitas Diponegoro, Semarang.

Dirgantara, S., A. Nawawi dan M. Insanu. 2013. Uji aktivitas antioksidan tiga spesies tanaman sarang semut (Famili: Rubiaceae) asal Kabupaten Merauke, Papua. J. Biol. Papua. 5(1):10-16.

Doyle, A. and J.B. Griffiths. 2000. Cell and tissue culture for medicinal research. John Willey and Sons. New York.

Ernawati dan H. Susanti. 2014. Penghambatan aktivitas xanthine oxidase oleh ekstrak etanol sarang semut (Myrmecodia tuberosa (non Jack) Bl.) secara in vitro. J. Pharmaçiana. 4(1): 15–22.

Frengki, Roslizawaty dan D. Pertiwi. 2014. Uji toksisitas ekstrak etanol sarang semut lokal Aceh (Mymercodia sp.) dengan metode BSLT terhadap larva udang Artemia salina Leach. J. Medika Veterinaria. 8(1): 60–62.

Geran, R.I., N.H. Greenberg, M.M. MacDonald, A.M. Schumacher and B.J. Abbott. 1992. Protocol for screening chemical agents and natural product against animal tumors and Other Biological System. Cancer Chemother. 3: 59–61.

Harborne, J.B. 1987. Metode fitokimia: Penuntun cara modern menganalisis tumbuhan. Penerjemah K. Padmawinata & I. Sudiro. Institut Teknologi Bandung, Bandung.

Hertiani, T., E. Sasmito, Sumardi and M. Ulfah. 2010. Preliminary study on immunomodulatory effect of sarang semut tubers Myrmecodia tuberosa and Myrmecodia pendens. OnLine J. Biol. Sci. 10: 136-141.

Meyer, B.N., N.R. Ferigni, J.E. Putnam, L.B. Jaconsen, D.F. Nichols, and J.L. McLaughlin. 1982. Brine shrimp a convenient general bioassay for active plant constituents. Planta Medica. 45: 31–34.

Middelton, E., C. Kandaswami and T.C. Theoharides. 2000. The effect of plant flavonoids on mammalian cells implications for inflammation, heart disease and cancer. Pharmacological Review. 52(4): 673–751.

Rachman, A.A. 2006. Senyawa aktif bersarang di sarang semut. Majalah Natural. 18-19.

Raineri, R., J.A. Poiley, R.J. Pienta and A.W. Andrews. 1981. Metabolic activation of carcinogens in the Salmonella mutagenicity assay by hamster and rat liver S-9 preparations. Environmental and Molecular Mutagenesis. 3(1): 71–84.

Rang, L.Y., A. Camerlengo, A.K.A. Wahab, and N. Mokhtar. 2003. Interannual variability of certain meteorological parameters in East Malaysia. Part II. J. Science International. 15(2): 147–150.

Rita, W.S. 2008. Isolasi dan identifikasi senyawa yang berpotensi sebagai antitumor pada daging buah pare (Momordica charantia L). J. Kimia. 2(2): 6–11.

Robinson, T. 1991. Kandungan organik tumbuhan tinggi. (Penerjemah: K. Padmawinata). ITB, Bandung.

Sharma, V. and R. Paliwal. 2013. Isolation and characterization of saponins from Moringa Oleifera (Moringaeceae) Pods. International Journal of Pharmacy and Pharmaceutical Sciences. 5(1): 179-183.

Shashi, B.M. and K.N. Ashoke. 1991. Tripenoid saponins discovered between 1987 and 1989. Phytochemistry. 30(5): 1357-1385.

Soeksmanto, A., M.A. Subroto, H. Wijaya and P. Simanjuntak. 2010. Anticancer activity for extracts of sarang semut plant (Myrmecodia pendens) to HeLa and MCM-B2 Cells. Pakistan J. Biol. Sci. 13: 148-151.

Soeksmanto, A., P. Simanjuntak dan M.A. Subroto. 2009. Uji toksisitas akut ekstrak air sarang semut (Myrmecodia pendans) terhadap histologi organ hati mencit. J. Nature Indonesia. 12(2): 152–155.

Sorgeloos, P., C. Remiche and G. Persoone. 1978. The use of Artemia salina Leach for toxicity tests. Ecotoxicology and Environmental Safety. 2: 249-255.

Subroto, M.A. dan H. Saputro. 2006. Gempur penyakit dengan sarang semut. Penerbit Swadaya, Jakarta.

Downloads

Published

2018-05-13

Issue

Vol. 8 No. 1 (2016)

Section

Research Articles

License

1. Copyright Policy

Jurnal Biologi Papua is committed to promoting the dissemination, accessibility, and responsible reuse of scholarly research in biology and related disciplines.

Under this policy, authors retain copyright ownership of their published articles and grant Jurnal Biologi Papua the non-exclusive right of first publication.

Authors retain the right to use, distribute, reproduce, and share their scholarly work in accordance with the applicable publication license.

The journal acknowledges the intellectual contributions of authors and respects their moral rights, including the right to be properly identified as creators of their work.

2. Publication License

Articles published under this policy are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).

License URL: https://creativecommons.org/licenses/by/4.0/

Under this license, users are permitted to:

  1. Share: Copy and redistribute published articles in any medium or format.

  2. Adapt: Remix, transform, and build upon published materials for any purpose, including commercial purposes.

  3. Redistribute: Make articles available through repositories, websites, databases, and other scholarly platforms.

These permissions are subject to the Creative Commons Attribution 4.0 International License.

Users must provide appropriate credit to the original authors, include a link to the license, and indicate whether changes have been made.

Reuse must not imply endorsement by the authors or publisher.

3. Rights of Authors

Authors publishing in Jurnal Biologi Papua retain the following rights:

  1. Copyright ownership of their original scholarly work.

  2. The right to distribute and share their published articles.

  3. The right to deposit their articles in institutional or subject repositories.

  4. The right to reuse their work in subsequent scholarly publications, subject to appropriate acknowledgment and applicable ethical standards.

  5. The right to share their work for teaching, research, academic collaboration, and other lawful purposes.

Authors must acknowledge the original publication in Jurnal Biologi Papua when distributing or reusing the published version.

4. Rights of the Publisher

Authors grant Jurnal Biologi Papua the non-exclusive right to publish, disseminate, archive, and preserve accepted manuscripts.

The journal may reproduce and distribute the published version through its official website and authorized indexing, archiving, and scholarly dissemination services.

The publisher is responsible for maintaining the integrity of published content and accurately displaying copyright and licensing information.

5. Open Access Policy

Jurnal Biologi Papua supports immediate open access to scholarly research.

Articles published under this policy are freely accessible to readers without subscription requirements or access fees.

The journal encourages the responsible dissemination and reuse of published research to support scientific progress, academic collaboration, and the broader exchange of knowledge.

Published content may be accessed, downloaded, shared, and reused in accordance with the applicable Creative Commons license.

6. Third-Party Materials

Authors are responsible for ensuring that all materials included in their manuscripts comply with applicable copyright and intellectual property laws.

Permission must be obtained when required for the use of third-party figures, photographs, tables, illustrations, or other copyrighted materials.

Materials excluded from the article's Creative Commons license must be clearly identified with their applicable copyright and permission notices.

The journal's publication license does not override the rights of third-party copyright holders.

7. Repository and Self-Archiving Policy

Authors are permitted and encouraged to deposit and share their published articles through institutional repositories, academic repositories, and personal or institutional websites.

Authors must provide appropriate acknowledgment of the article's original publication in Jurnal Biologi Papua, including its citation information and DOI where available.

Repository deposits and subsequent distribution must comply with the license applicable to the relevant version of the article.

8. Licensing Information and Article Metadata

The applicable copyright holder and publication license should be clearly identified on each published article's webpage.

Where supported, licensing information should also be included in article metadata and the published PDF.

The journal is committed to maintaining accurate and consistent licensing information across its online publishing platform and scholarly indexing services.

9. Policy Application and Previously Published Articles

This policy applies to manuscripts accepted following its formal adoption by the journal and publisher.

Articles published before the adoption of this policy remain subject to the copyright arrangements and licenses applicable at the time of their publication, unless a valid change is authorized by the relevant rights holders.

The journal will not apply a new license retroactively without the necessary authorization.

10. Policy Commitment

Jurnal Biologi Papua is committed to transparent copyright arrangements, responsible open access publishing, and respect for intellectual property rights.

The journal will periodically review its copyright and licensing practices to maintain consistency with recognized standards of scholarly communication.

References

Creative Commons Attribution 4.0 International License: https://creativecommons.org/licenses/by/4.0/

Directory of Open Access Journals (DOAJ): https://doaj.org/apply/copyright-and-licensing/