Analisis Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus musculus L.) Swiss Webster Setelah Induksi Ochratoxin A Selama Periode Organogenesis

Arum Setiawan, Mammed Sagi, Widya Asmara, Istriyati Istriyati


The aims of this study were determined the effects of Ochratoxin A (OTA) on growth of fetus tibia epiphyseal cartilage during organogenesis period. Twenty four pregnant mice were divided randomly into 4 groups of 6. Ochratoxin A was dissolved in sodium bicarbonateand administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining were used as control. The fetal tibia was taken after the 18 th day of pregnancy. The growth of tibia epiphyseal cartilages were observed histologically using Erlich’s Haematoxylin-Eosin Stain. The result of this study indicated that OTA caused decreased thickness of the rest zone, proliferative zone, maturation zone and calsification zone of the fetus tibial growth plate significantly. 

Key words: Ochratoxin A, tibia, cartilage, and thickness.

Full Text:



Fung, F. and R.F. Clark. 2004. Health effects of mycotoxins: a toxicological overview. J. Toxicol. 42(2): 217-234.

Gilbert, S.F. 1991. Developmental Biology. 3rd ed. Sinauer Associates Inc. Publishers, Massachussets, 200–275.

Hoehler, D., R.R. Marquardt, A.R. McIntosh and G.M. Hatch. 1997. Induction of free radicals in hepatocytes, mitochondria and microsomes of rats by ochratoxin A and it’s analogues. Biochim. Biophys. Acta. 1357: 225–233.

Junqueira, L.C., J. Carneiro and R.O. Kelley. 1998. Histologi Dasar. Alih Bahasa oleh J. Tambayong. Penerbit Buku Kedokteran EGC. Jakarta. hal. 143–150.

Khan, S., M. Martin, H. Bartsh and A.D. Rahimtula. 1989. Perturbation of liver microsomal calcium homeostasis by ochratoxin A. Biochem. Pharmacol. 38: 67–72.

Langman, S. 2009. Medical embryology, human development normal and abnormal. 11th ed. The Williams and Witkisn Co.

Marasas, W.F.O. and P.E. Nelson. 1987. Mycotoxicology ‘introduction to the mycology, plant pathology, chemistry, toxicology, and pathology of naturally occurring mycotoxicoses in animals and man’. The Pennsylvania State University Press, University Park and London. USA.

Marti, N.B. 2006. Ochratoxin A and ochratoxigenic modulds in grapes, must and wine, ecophysiological study, tesis doctoral Universitat de Lleida Spain, from URL: 10 Juni 2007.

Miraglia, M. and C. Brera. 2002. Assessment of dietary intake of ochratoxin A by the population of EU member states. Directorate General Health and Consumer Product. Rome, Italy.

Ohta, K., M. Maekawa, R. Katagiri, E. Ueta and I. Naruse. 2006. Genetic susceptibility in the neural tube defects induced by ochratoxin A in the genetic archiencephaly mouse, Pdn/Pdn, Congen. Anom. Kyo. 46: 144–148.

Sava, V., O. Reunova, A. Velasques, R. Harbison and J. Sanchez-Ramos. 2006. Can low level exposure to ochratoxin A cause parkinsonism. J. Neurol Sci. 249: 68–75.

Setiawan, A., M. Sagi, W. Asmara dan Istriyati. 2011. Analisis kuantitatif sel purkinje cerebellum mencit (Mus musculus L.) Swiss Webster setelah induksi ochratoxin A selama periode organogenesis. J. Biota 16(2): 262–268.

Setiawan, A. 2002. Pengaruh morfin terhadap pertumbuhan kartilago epifisialis os tibia fetus mencit (Mus musculus L.). J. Pen. Sains 12: 9–16.

Suntoro, S.H. 1983. Metode pewarnaan (histologi dan histo-kimia). Penerbit Bharata Karya Aksara. Jakarta. 48-72.

Tuchmann–Duplessis, H. 1975. Drug effects on the fetus. Adis Press, New York. 9–82.

Ueta, E., M. Kodama, Y. Sumino, M. Kurome, K. Ohta, R. Katagiri and I. Naruse. 2009. Gender-dependent dif-ferences in the incidence of ochratoxin A-induced neural tube defects in Pdn/Pdn mouse. Con. Anom. Manuscript ID :CGA-08-2009-043.R2.

Wangikar, P.B., P. Dwivedi and N. Sinha. 2004. Effects in rats of simultaneous prenatal exposures to ochratoxin A and aflatoxin B1.I. Maternal toxicity and fetal malformation, Birth Defects Res. (Part B). 71: 343–351.

Zhang, X., C. Boesch-Saadatmandi, Y. Lou, S. Wolfram, P. Huebbe and G. Rimbach. 2009. Ochratoxin A induces apoptosis in neuronal cells. Genes. Nutr. 4: 41–48.



  • There are currently no refbacks.