Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)


  • Wijanarka Wijanarka Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang
  • Sri Pujiyanto Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang
  • Budi Raharjo Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang



Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens.  

Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.


Download data is not yet available.

Author Biographies

Wijanarka Wijanarka, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang

Biologi Departemen, Fakultas Sains dan Matematika Universitas Diponegoro Semarang

Sri Pujiyanto, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang

PS. Biologi Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang

Budi Raharjo, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang

Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang


Ahuja, M.R. l982. Isolation, culture and fusion of protoplast: problems and prospects. Silvae Genetica. 31: 66–77.

Al-Arif, M.A, W. Darmanto dan N.N.T. Nurhajati. 2012. Isolasi dan identifikasi bakteri selulolitik dengan aktivitas tinggi dalam saluran pencernaan keong emas (Pomacea canaliculata). Jurnal JBP Biosains. 14(2): 86-92.

Alexander, M.A. and T.W. Jeffries. 1990. Respiratory efficiency and metabolize partitioning as regulatory phenomenain yeasts. Enzyme Microbe. Technol. 12: 2-29.

Allais, J.J., S. Kammou, P. Blanc, C. Girard and J. Baratti. 1986. Isolation and characteristic of bacterial strains with inulinase activity. Appl. Environ. Microbiol. 52(50): 1086-1090.

Baltz, R.H. l996. Strain Improvement. In: Manual of industrial microbiology and biotechnology. Demain, A.I., and Solomon, N.A. (Ed). American Society for Microbiology. Washington.

Barnet, J.A., R.W. Payne and D. Yarrow. 1990. Yeast characteristics and identification. Cambridge University Press.

Bonciu, C., V. Struta and G. Bahrim. 2010. Isolation and screening of new mould srains able for inulinase biosintesis and inulin from Jerusalem Artichoke hydrolysis. Innov. Rom. Food. Biotechnol. 7: 77–81.

Castro, V.A., E.A. Albrecht, I.A. Vega, E. Koch and C. Gamarra-Luques. 2002. Pigmented corpuscles in the midgut gland of Pomacea canaliculata and other neotropical apple snails (Prosobrancgia, Ampullariidae). Possible Symbiotic Association. Biocell. 26(1): 101-109.

Chaplin, M.F and J.F. Kennedy. 1994. Carbohydrat analysis: A practical approach. 2nd Edition. Oxford University Press. Oxford.

Ezeronye, O.U and P.O. Okerentugba. 2001. Optimum conditions for yeast protoplast release and regeneration in S. cerevisiae and C. tropicalis using gut enzyme of the Giant African Snail Achatina achatina. Letters in Applied Microbiology. 32: 190-193.

Farahnak, F., T. Seki, Y.R. Dedwey, and D. Ogrydziak. 1986. Contruction of lactose-assimilating and high ethanol producing yeast by protoplast fusion. Applied and environmental microbiology. 51(2): 362-367.

Guo, N., F. Gong, Z. Chi, J. Sheng and J. Li. 2009. Enhanced inulinase production in solid state fermentation by a mutant of the marine yeast Pichia guilliermondii using surface respone methodology and inulin hydrolysis. J. Ind. Microbiol. Biotechnol. 36: 499-507.

Haddad, R., Alemzadeh, E., Ahmadi, A. R., Hosseini, R., Moezzi, M. 2017. Identification of chlorophyceae based on 18S rDNA sequences from Persian Gulf. Iran. J Microbiol. 6(6): 437-442.

Hikmatyar, M.F., Royani, J.I., and Dasumiati. 2015. Isolasi dan amplifikasi DNA Keladi Tikus (Thyponium flagelliform) untuk identifikasi keragaman genetik. J. Bioteknol. Bios. Indon. 2(2): 42-48.

Holz, G., and G. Saunders. l985. Genetic modification of industrial microorganisms. In: Comprehensive biotechnology. The principles, application and regulation of biotechnology in industry, agryculture and medicine. Moo-Young, M (Ed) Pergamon Press.

Isci, B., H.K. Yildirim, and A. Altindisli. 2014. Evaluation of methods for DNA extraction from Must and Wine. J. Inst. Brew. 120: 238–243.

Javadekar, V.S., H.S. Raman, and D. Gokhale. 1995. Industrial yeast strain improvement: Construction of a Highly flocculent yeast with a killer character by protoplast fusion. Jour. Indst. Microbiology. 15: 94-102.

Kierstan, M.P.J. l980. Production of syrup from inulin containing plants. J. Biotech. Bioeng. 20: 447-450.

Kumar, N. S., and G. Gurusubramanian. 2011. Random Amplified Polymorphic DNA (RAPD) markers and ITS applications. Sci. Vis. 11(3): 116-124.

Li, L., J. Frohlich, P. Pfeiffer, and H. Konig. 2003. Termite gut symbiotic archaezoa are becoming living metabolic fossils. Eukaryotic Cell. 2(5): 1091-1098.

Lunggani, A. T., Wijanarka, dan K. Endang. 2009. Produksi IOS prebiotik berbasis pemanfaatan Umbi Dahlia (Dahlia variabilis) oleh khamir inulinolitik dan pengujian antimikrobanya secara in vitro. Penelitian Hibah Multiyears Desentralisasi. Undip Semarang.

Matsushima, R., and R.H. Baltz. l986. Protoplast fusion. In: Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology. Washington DC.

Park, J.P and J.W. Yun. 2001. Utilization of chicory roots for microbial endoinulinase production.Letters In Applied Microbiology. (33): 183–187.

Rouwenhorst, R.J., M. Hensing, J. Verbakel, W.A. Scheffer, and J.P. van Dijken. 1990b. Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 56(11): 3337–3345.

Rouwenhorst, R.J. L.E. Visser, A.A van Derbaan, W.A. Scheffer and J.P. van Dijken. 1988. Production, distribution and kinetic properties of inulinase in continous culture of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 54(5): 1131-1137.

Sahin, S., I. Osman, and H.H. Biyik. 2013. Purification and caracterization of endo-β-1-4-glucanase from local isolate Trichoderma ouroviride. International Journal of Bioscience, Biochemistry and Bioinformatic. 3(2): 129-132.

Sandy, Y.A., S. Djauhari, dan A.W. Sektiono. 2015. Indentifikasi molekuler jamur antagonis Trichoderma harzianum di isolasi dari tanah pertanian di Malang Jawa Timur. Jurnal HPT 3(3): 1–8.

Santiago, C.M. l982. Protoplast fusion a new tecnique for genetic manipulation and breeding of industrial micro-organisme. I.C. Biotech. 5: 435-440.

Santopietro, L.M.D., JFT. Spencer, D.M. Spencer and Sineriz. 1997. Characterization of intergeneric hybrids obtained by protoplast fusion between Phaffia rhodozyma, Cryptococcus laurentii and S. cerevisiae. Biotechnology technique. 11(10): 769-771.

Sena, A., G.L.V. Junior., A.G. Neto., A.G. Taranto, C. P. Pirovani., J.C.M. Cascardo., R.B. Zingali., M.A. Bezerra, and S. Assis. 2011. Production, purification and characterization of a thermostable β-1-3 glucanase (Laminarinase) produced By Moniliophthora perniciosa. An Acad Bras Science. 83(2): 599-609.

Stratford, M. 2006. 3374-Pichia mandshurica. national collection of yeast cultures. UK. 23 Februari 2010.

Sumerta, I.N., dan A. Kanti. 2017. Keragaman jenis khamir penghasil etanol yang di isolasi dari makanan fermentasi di Kepulauan Riau. Jurnal Biologi Indonesia 13(1): 61-69.

Verma, N., Bansal and K. Vivek. 2004. Protoplast fusion technology and its biotechnology Applications.

Wei, W., Kunlu Wu, Yan Qin, Zhong Xie and Xinsheng Zhu. 2001. Intergeneric protoplast fusion between Kluyveromyces and S. cerevisiae to produce sorbitol from Jerusalem Artichokes. Biotechnology Letters 23: 799-803.

Wenzel, M., I. Schonig, M. Berchtold, P. Kamfer, and H. Konig. 2002. Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopis angusticollis. Journal of Applied Microbiology. 92: 32-40.

Wijanarka, E. Arina T.L. dan H.P. Sakti. 2005. Fusi protoplas interspesifik termostabil Kluveromyces marxianus dan Torulospora pretoriensis isolat lokal serta aplikasinya pada HFS. Prosiding Workshop/Seminar Hasil Penelitian Propinsi Jawa Tengah. 2005.

Wijanarka, Endang, Hermin, PS. 2006. Eksplorasi khamir inulinolitik termostabil umbi Dahlia (Dahlia variabilis Willd) Jawa Tengah. Laporan Hibah Bersaing.

Wijanarka. 2016. Eksplorasi bakteri glukanolitik indigenous dari saluran pencernaan Bekicot (Achatina fulica) sebagai biokatalis isolasi protoplas dan aplikasinya pada fusi protoplas Pichia manshurica DUCC-Y15 untuk meningkatkan produksi inulinase. Laporan Hasil Penelitian Fundamental (Tidak dipublikasikan). Universitas Diponegoro, Semarang.

Wijanarka., T.L. Arina, dan Y. Emi. 2001. Seleksi khamir dan optimasi produksi enzim inulinase dari tanah sekitar Umbi Ketela Rambat (Ipomoea batatas) di daerah Bandungan Ambarawa. Penelitian BBI Dosen Muda Tahun 2001.

Xiao, R., M. Tanida, dan S. Takao. 1998. Inulinase from Crysosporium pannorum. J. Ferment. Technol. 66(5): 244–248.

Xiao, R., M. Tanida, and S. Takao. 1989. Purification and some properties of endoinulinase from Crysosporium pannorum. J. Ferment. Bioeng. 67(4): 244-245.






Research Articles