Skrining Fitokimia dan Uji Antioksidan Ekstrak Biji Kopi Sangrai Jenis Arabika (Coffea arabica) Asal Wamena dan Moanemani, Papua

https://doi.org/10.31957/jbp.925

Authors

  • Septiani Mangiwa Universitas Cenderawasih
  • Agnes Eri Maryuni Cenderawasih University

Abstract

Coffee bean are rich of secondary metabolits that able to inhibit free radical compounds. This antioxidant activity may reduce many diseases correlated with it. The aims of this study were to determined the phytochemical content and antioxidant activity of roasted coffee bean from Wamena and Moenemani regency, Papua. Roasted coffee beans were extracted by maceration for 24 hr with methanol. Harborne standard method was used for the phytochemical analysis  and DPPH assay was used to  determine the antioxidant activity. IC50 was measured by spectrophotometric assay using spectrophotometer Uv-Vis at 517 nm wavelenghth. Result showed that both Arabica roasted coffee beans from Wamena and Moanemany had the capacity to inhibit free radical  at 61,71% and 69,7% with IC50 at  107,97 and 100,91 ppm, respectively . Phytochemical investigation revealed that the bioactive compounds from Moanemani and Wamena coffee beans were similar, which composed of alkaloids, flavonoids, terpenoids, saponins, and tanins. In conclusion, the methanolic extract of roasted Arabica coffee beans from Wamena and Moanemani can be used as the source of natural antioxidant.

Keywords: Arabica roasted coffee beans; phytochemical; antioxidant; DPPH method.

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Author Biographies

Septiani Mangiwa, Universitas Cenderawasih

Biochemistry

Agnes Eri Maryuni, Cenderawasih University

Biochemistry

References

Belay, A., and A.V. Gholap. 2009. Characterization and determination of chlorogenic acid (CGA) in coffee beans by UV-Vis spectroscopy. African Journal of Pure and Applied Chemistry. 3(11): 234-240.

Bicho, N.C., A.E. Leitao, J.C. Rumalho, and F.C. Lidon. 2011. Identification of chemical clusters discriminator of roast degree in arabica and robusta coffee beans. Journal Europa Food Res Technology.

Cämmer, B., and L.W. Kroh. 2006. Antioxidant activity of coffee brews. Eur Food Res Technol. 223: 469-474.

Ewa, N., G. Budryn, and J. Kula. 2007. The effect of roasting method on headspace composition of Robusta coffee bean aroma. Eur Food Res Techol. 225 : 9-19.

Farah, A., T. Paulis, L. Trugo, and P.R. Martin. 2005. Effect roasting on the formation chlrogenic acid lactone in coffea. Journal Agricultural and Food Chemistry. 53: 1505-1513.

Harborne, J.B. 1996. Metode fitokimia: Penuntun cara modern menganalisis tumbuhan. Cetakan kedua. Penterjemah: K. Padmawinata & I. Soediro. Penerbit ITB Press. Bandung.

HeÄimović, I., A.B. Cvitanović, D. Horžić, and D. Komes. 2011. Comparative study of poliphenols and caffeine in different coffee varieties affected by degree of roasting. Food Chemistry. 129: 991–1000.

Marlina, D.S., V. Suryanti, dan Suyono., 2005. Skrining fitikimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq. Swart) dalam esktark etanol. Biofarmasi. 3(1): 26–31.

Mangiwa, S., A. Futwembun, dan P.M. Awak. 2014. Kadar asam klorogenat (CGA) dalam biji kopi arabika (Coffea arabica) asal Wamena, Papua. Jurnal Ilmiah Kependidikan Kimia "Hydrogen". 2(2): 313-317.

Mangiwa, S., dan A.E. Maryuni. 2015. Pengaruh suhu penyangraian terhadap kadar kafein dalam biji kopi arabika (Arabica coffea). Prosiding Seminar Hasil Penelitian Pengembangan Ipteks dan Sains, LPPM Uncen. Edisi 1, April 2015 : 7-11.

Mangiwa, S., dan Y.R. Yabansabra. 2016. Kadar trigonelin dalam biji kopi arabika (Coffea arabica) asal Wamena, Kabupaten Jayawijaya, Papua. Jurnal SAINS dan Pengajaranya. 16(1): 29-34.

Minamisawa, M, S. Yoshida, and N. Takai. 2004. Determination of biollogically active substances in roasted cofees using a diode-array HPLC system. Analytical Science. 20: 325-328.

Molyneux, P. 2004. The use of the stable free radical diphenylpicrylhydrazil (DPPH) for estimating antioxidant activity. Songklanarin J. Sci. Technol. 26(2): 211-219.

Naidu, M.M., G. Sulochanamma, S.R. Sampathu, and P. Srinivas. 2008. Studies on extraction and antioxidant potential of green coffee. Food Chemistry. 107: 337-384.

Svehla, D. 1990. Vogel: Buku teks analisis anorganik kualitatif makro dan semimikro. Edisi Ke Lima. Penterjemah: Setiono dan S. Pujiadmaka, P. Hadyana. Penerbit Kalman Media Nusantara. Jakarta.

Tamilmani, P., and M.C. Pandey. 2015. Optimization and elaviation of phenolic coumpound and their antioxidant activity from coffee beans. International Journal of Advanced Research. 3(4): 296–306.

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Published

2019-10-31

Issue

Vol. 11 No. 2 (2019)

Section

Research Articles

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